Effect of acetate upon the formation of acetoin in Klebsiella and Enterobacter and it possible practical application in a rapid voges-proskauer test.
نویسندگان
چکیده
Acetate stimulates the formation of acetoin during 1-h incubation of Voges-Proskauer-positive strains of Klebsiella and Enterobacter. Of these organisms, 124 of 126 strains were recognized as positive in the presence of acetate, and 106 were recognized as positive in its absence.
منابع مشابه
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BARRITT, M. M. 1936 Intensification of the Voges-Proskauer reaction by the addition of alpha naphthol. J. Path. Bacteriol., 42, 441-454. EGGLETON, P., ELSDEN, S. R., AND GOUGH, N. 1943 The estimation of creatine and of diacetyl. Biochem. J., 37, 526-529. HELPRIN, J. J., AND SULLIVAN, M. C. 1954 The production of acetylmethylcarbinol by Aerobacter from a variety of carbon sources in a synthetic ...
متن کاملAnalysis of acetoin and diacetyl in bacterial culture supernatants by gas-liquid chromatography.
The acetoin and diacetyl contents of culture supernatants of Voges-Proskauer-positive "viridans" streptotocci, Klebsiella pneumoniae and Staphylococcus aureus, were determined by a gas liquid chromatographic procedure, in which supernatants were extracted with diethyl ether and diacetyl was measured on columns of 10% (wt/wt) polyethylene glycol 400 (PEG 400) at 73 C. Acetoin was converted to di...
متن کاملVoges-Proskauer Test with Leifson's Reagent
At. Most bacteriologists held that the old Voges?SKauer's test (1898) was not a satisfactory test for the differentiation of the coli-serogenes group of organisms. During carbohydrate fermentation by the aerogenes group of bacilli, acetoin (acetylmethyl carbinol) is formed, and this is detected by caustic potash and peptone. Acetoin is oxidized to diacetyl, and this reacts with caustic potash a...
متن کاملA Colorimetric Determination of Blood Acetoin” by W. W. Westerfeldt
Most of the methods described for the determination of acetoin and biacetyl are based on the original Lemoigne-van Niel procedure (1, 2), which depends upon the formation of insoluble nickel dimethylglyoxime when biacetyl is treated with hydroxylamine in the presence of a nickel salt; acetoin is similarly determined after oxidation to biacetyl by heating with ferric chloride. In a recent modifi...
متن کاملA colorimetric determination of blood acetoin.
Most of the methods described for the determination of acetoin and biacetyl are based on the original Lemoigne-van Niel procedure (1, 2), which depends upon the formation of insoluble nickel dimethylglyoxime when biacetyl is treated with hydroxylamine in the presence of a nickel salt; acetoin is similarly determined after oxidation to biacetyl by heating with ferric chloride. In a recent modifi...
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عنوان ژورنال:
- Applied microbiology
دوره 25 3 شماره
صفحات -
تاریخ انتشار 1973